Analysis of the Effectiveness of Chrysomya sp. Maggot Extract in Inhibiting the icaA and icaD Genes Regulator Staphylococcus epidermidis Biofilm

Dylan Tamalsir, Anton Budhi Darmawan, Dwi Utami Anjarwati

2020

Abstract

Maggots have proven to be one of the significant ingredients in degrading biofilms by destroying the polysaccharide intercellular adhesion (PIA) produced by the enzyme encoded by the icaADBC gene. This experimental study aims to prove the action target of maggot Chrysomya sp. extract on icaAD biofilm regulatory genes. Biofilm DNA extraction was carried out against bacterial cultures of S. epidermidis ATCC 35984 and S. epidermidis TCC 35983 incubated with Chrysomya sp. maggot extract, using Microtiter plate (MTP) method. The Chrysomya sp. maggot extract concentration was 0%, 20%, 40%, 60%, 80%, and 100% with an incubation period of 3 and 24 hours. PCR analyzed gene expression with the primer of icaAD genes. The qualitative test was carried out by 2% agarose gel electrophoresis. IcaAD genes of both S. epidermidis strains were detected in all treatments. The icaA band size of ± 980 bp and the icaD gene size of ± 380 bp can be observed either after the intervention of Chrysomya sp. maggot extract in various concentration (0%, 20%, 40%, 60%, 80%, and 100%) or after the incubation period of 3 and 24 hours. Chrysomya sp. maggot extract does not affect the icaAD biofilm regulatory genes of Staphylococcus epidermidis.

Paper Citation

in Harvard Style

Tamalsir D., Darmawan A. and Anjarwati D. (2020). Analysis of the Effectiveness of Chrysomya sp. Maggot Extract in Inhibiting the icaA and icaD Genes Regulator Staphylococcus epidermidis Biofilm.In Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC, ISBN 978-989-758-499-2, pages 121-125. DOI: 10.5220/0010488801210125

in Bibtex Style

@conference{jimc20,
author={Dylan Tamalsir and Anton Budhi Darmawan and Dwi Utami Anjarwati},
title={Analysis of the Effectiveness of Chrysomya sp. Maggot Extract in Inhibiting the icaA and icaD Genes Regulator Staphylococcus epidermidis Biofilm},
booktitle={Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,},
year={2020},
pages={121-125},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010488801210125},
isbn={978-989-758-499-2},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st Jenderal Soedirman International Medical Conference in conjunction with the 5th Annual Scientific Meeting (Temilnas) Consortium of Biomedical Science Indonesia - Volume 1: JIMC,
TI - Analysis of the Effectiveness of Chrysomya sp. Maggot Extract in Inhibiting the icaA and icaD Genes Regulator Staphylococcus epidermidis Biofilm
SN - 978-989-758-499-2
AU - Tamalsir D.
AU - Darmawan A.
AU - Anjarwati D.
PY - 2020
SP - 121
EP - 125
DO - 10.5220/0010488801210125