Wharton’s Jelly Mesenchymal Stem Cells-secreted IDO as Candidate of Anti-inflammation Therapy

Wahyu Widowati, Teresa Liliana Wargasetia, Fanny Rahardja, Rimonta Gunanegara, Hanna Kusuma, Seila Arumwardana, Cintani Wahyuni, Cahyaning Wijayanti, Tri Handayani, Rizal Rizal, Rizal Rizal

2021

Abstract

Local inflammation in lung can induce by viral pneumonia which causes acute respiratory distress syndrome (ARDS). ARDS also caused by COVID-19 SARS-COV-2 infection. hWJMSCs will release anti-inflammatory signals such as indoleamine 2,3 dioxygenase (IDO) as tissue homeostasis between MSCs and resident macrophages as anti-inflammatory signals. This led to the idea of investigating potential of hWJMSCs-Secreted IDO as candidate of anti-inflammation therapy. The hWJMSCs have been isolated from the human umbilical cord using an explant method and characterized using a flow cytometer to detect the cell surface markers CD105, CD73, CD44, CD90, and negative lineage expression of hWJMSCs. The hWJMSCs secretome was characterized by measuring the level of indoleamine 2,3-dioxygenase (IDO) in various starving cells. The isolated umbilical cord hWJMSCs showed positive expression of CD105, CD73, CD44, CD90, and negative lineage expression from 5, 10, and 15 passage. The hWJMSCs IDO secretion level was 5.86 ng/mL for non-starving cells, 6.84 ng/mL for 24 h starving cells, 9.59 ng/mL for 48 h starving cells, and 13.32 ng/mL for 72 h starving cells. The early, medium, and old passage of hWJMSCs have the same characteristics. Longer starvation periods up to regulate the IDO level in hWJMSCs secretome which indicate as anti-inflammation therapy.

Paper Citation

in Harvard Style

Widowati W., Liliana Wargasetia T., Rahardja F., Gunanegara R., Kusuma H., Arumwardana S., Wahyuni C., Wijayanti C., Handayani T. and Rizal R. (2021). Wharton’s Jelly Mesenchymal Stem Cells-secreted IDO as Candidate of Anti-inflammation Therapy. In Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES, ISBN 978-989-758-601-9, pages 271-278. DOI: 10.5220/0010749700003113

in Bibtex Style

@conference{ice-tes21,
author={Wahyu Widowati and Teresa Liliana Wargasetia and Fanny Rahardja and Rimonta Gunanegara and Hanna Kusuma and Seila Arumwardana and Cintani Wahyuni and Cahyaning Wijayanti and Tri Handayani and Rizal Rizal},
title={Wharton’s Jelly Mesenchymal Stem Cells-secreted IDO as Candidate of Anti-inflammation Therapy},
booktitle={Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,},
year={2021},
pages={271-278},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010749700003113},
isbn={978-989-758-601-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,
TI - Wharton’s Jelly Mesenchymal Stem Cells-secreted IDO as Candidate of Anti-inflammation Therapy
SN - 978-989-758-601-9
AU - Widowati W.
AU - Liliana Wargasetia T.
AU - Rahardja F.
AU - Gunanegara R.
AU - Kusuma H.
AU - Arumwardana S.
AU - Wahyuni C.
AU - Wijayanti C.
AU - Handayani T.
AU - Rizal R.
PY - 2021
SP - 271
EP - 278
DO - 10.5220/0010749700003113