Effects of Herbal Ingredients (Allium sativum, Punica granatum, Curcuma longa, Curcuma xanthorrhiza) on FATP3 Gene Expression in Aorta of High Fat Diet-fed Rats: A Preliminary Study

Diana Krisanti Jasaputra, Diana Krisanti Jasaputra, Julia Windi Gunadi, Julia Windi Gunadi, Penny Setyawati Martioso, Larissa, Yenny Noor, Irna Permanasari Gani, Erik Dwikurnia Saiman, Desman Situmorang, Andi Haryanto

2021

Abstract

: FATP (Fatty Acid Transport Protein) is a protein that facilitates uptake of LCFA (Long Chain Fatty Acid) by activating it into CoA-thioester and trapping them in the cell. FATP3 is critical for LCFA uptake in endothelial cells. Herbal ingredients are well known as anti-hyperlipidemic and anti-atherosclerotic agents, but the molecular mechanism for these effects are still unclear. Twenty-eight male Wistar rats used in this study were divided into negative control, positive control (HFD), and treatments (175 mg/kg BW Allium sativum, Punica granatum, Curcuma longa, Curcuma xanthorrhiza, and 1.8 mg/kg BW Rosuvastatin), each group consisted of 4 rats. The rats were given vitamin D 700.000 mg/kg BW single dose to all groups except for negative control, continued with HFD combined with herbal ingredients for twelve weeks. After treatments, the rats were sacrificed, RNA was extracted from the aorta to perform semi-quantitative PCR (FATP3 and GAPDH). We found no significant differences in FATP3 gene expression between all groups. In summary, herbal ingredients (Allium sativum, Punica granatum, Curcuma longa, Curcuma xanthorrhiza) do not influence FATP3 gene expression in the aorta of high fat diet-fed rats.

Paper Citation

in Harvard Style

Jasaputra D., Gunadi J., Martioso P., Larissa., Noor Y., Gani I., Saiman E., Situmorang D. and Haryanto A. (2021). Effects of Herbal Ingredients (Allium sativum, Punica granatum, Curcuma longa, Curcuma xanthorrhiza) on FATP3 Gene Expression in Aorta of High Fat Diet-fed Rats: A Preliminary Study. In Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES, ISBN 978-989-758-601-9, pages 328-332. DOI: 10.5220/0010753800003113

in Bibtex Style

@conference{ice-tes21,
author={Diana Krisanti Jasaputra and Julia Windi Gunadi and Penny Setyawati Martioso and Larissa and Yenny Noor and Irna Permanasari Gani and Erik Dwikurnia Saiman and Desman Situmorang and Andi Haryanto},
title={Effects of Herbal Ingredients (Allium sativum, Punica granatum, Curcuma longa, Curcuma xanthorrhiza) on FATP3 Gene Expression in Aorta of High Fat Diet-fed Rats: A Preliminary Study},
booktitle={Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,},
year={2021},
pages={328-332},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010753800003113},
isbn={978-989-758-601-9},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 1st International Conference on Emerging Issues in Technology, Engineering and Science - Volume 1: ICE-TES,
TI - Effects of Herbal Ingredients (Allium sativum, Punica granatum, Curcuma longa, Curcuma xanthorrhiza) on FATP3 Gene Expression in Aorta of High Fat Diet-fed Rats: A Preliminary Study
SN - 978-989-758-601-9
AU - Jasaputra D.
AU - Gunadi J.
AU - Martioso P.
AU - Larissa.
AU - Noor Y.
AU - Gani I.
AU - Saiman E.
AU - Situmorang D.
AU - Haryanto A.
PY - 2021
SP - 328
EP - 332
DO - 10.5220/0010753800003113