Cloning, Expression and Sequence Analysis of PbeHSFA1d in Pyrus betulifolia

Cong Jin, QiaoHui Guo

2022

Abstract

Heat shock protein is a kind of transcription factors widely existing in plants, which plays a major role in regulating plant growth and responses to environmental stress. In order to study the sequence characteristics of HSF gene from pear and its response to abiotic stress, PbeHSFA1d gene was isolated from Pyrus betulifolia by RT-PCR. Sequence analysis of PbeHSFA1d was carried out by bioinformatics software, and real time quantitative PCR was used to detect the expression level of PbeHSFA1din different tissues and its response patterns to various abiotic stresses. The results reflected that the ORF of PbeHSFA1d was 1548 bp in length, 516 amino acids were encoded, and there were 52 phosphorylation sites in the predicted protein, but the glycosylation site was not found. Domain analysis showed that PbeHSFA1d contained a conserved N-terminal DNA binding domain (DBD), a bipartite oligomerization domain (HR-A/B), an activation domain (AHA), a nuclear localization signal (NLS) and a nuclear export signal (NES), which are typical structural features of plant HSF protein family. Moreover, PbeHSFA1d was close to MdHSFA1d, PaHSF8, PpHSF8 and PdHSFA1d in genetic relationship. PbeHSFA1d was localized to the nucleus by subcellular localization. The results of RT-PCR demonstrated that the expression of PbeHSFA1d in stem was higher than that in flower, leaf and root, and the transcript levels of PbeHSFA1d were induced by high temperature, dehydration, low temperature and salt. The results of this study will provide a theoretical basis for further exploration of the function in abiotic stress of PbeHSFA1d.

Paper Citation

in Harvard Style

Jin C. and Guo Q. (2022). Cloning, Expression and Sequence Analysis of PbeHSFA1d in Pyrus betulifolia. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 277-282. DOI: 10.5220/0011200700003443

in Bibtex Style

@conference{icbeb22,
author={Cong Jin and QiaoHui Guo},
title={Cloning, Expression and Sequence Analysis of PbeHSFA1d in Pyrus betulifolia},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={277-282},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011200700003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - Cloning, Expression and Sequence Analysis of PbeHSFA1d in Pyrus betulifolia
SN - 978-989-758-595-1
AU - Jin C.
AU - Guo Q.
PY - 2022
SP - 277
EP - 282
DO - 10.5220/0011200700003443