Knockdown of the Cysteine Dioxygenase Gene in Damaged Mitochondrial Cardiomyocytes May Be Protected by the Effective Range of Taurine Level

Lifan Zhou, Xinyi Zhou, Yanxi Ren, Yuwei Zha, Jiarong Bei

2022

Abstract

Cardiomyopathy is a disease caused by various reasons, including myocardial hypertrophy and autologous gene defects. Taurine has been used in cardiomyopathy treatment for many years. It is a cytoprotective agent that can protect the damaged mitochondria of the cardiomyocytes. Previous studies suggest that the high level of taurine is primarily controlled by the cysteine dioxygenase (CDO) gene. This paper investigates the optimum value of taurine concentration into CDO knockdown cardiomyocytes in vitro and vivo. In vitro, taurine solution (ranges from 0μL to 1000μL) was added into the CDO knockdown cell culture medium daily for every six groups. Then，we use ATP detection and Mitochondria membrane potential (MMP) detection to investigate the effect of the taurine supplement. In vivo, 50 male mice are divided into ten groups fed with taurine ranging from 1.0g to 3.0g as 0.2g increments. There is no result since all experiments are conducted in virtual due to COVID-19. However, this paper is the first that provides the protocol to detect the right taurine concentration in the case of CDO knockdown in cardiomyocytes. Thus, it may provide some ideas in treating cardiomyopathy using a suitable amount of taurine in clinical.

Paper Citation

in Harvard Style

Zhou L., Zhou X., Ren Y., Zha Y. and Bei J. (2022). Knockdown of the Cysteine Dioxygenase Gene in Damaged Mitochondrial Cardiomyocytes May Be Protected by the Effective Range of Taurine Level. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 944-950. DOI: 10.5220/0011313400003443

in Bibtex Style

@conference{icbeb22,
author={Lifan Zhou and Xinyi Zhou and Yanxi Ren and Yuwei Zha and Jiarong Bei},
title={Knockdown of the Cysteine Dioxygenase Gene in Damaged Mitochondrial Cardiomyocytes May Be Protected by the Effective Range of Taurine Level},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={944-950},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011313400003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - Knockdown of the Cysteine Dioxygenase Gene in Damaged Mitochondrial Cardiomyocytes May Be Protected by the Effective Range of Taurine Level
SN - 978-989-758-595-1
AU - Zhou L.
AU - Zhou X.
AU - Ren Y.
AU - Zha Y.
AU - Bei J.
PY - 2022
SP - 944
EP - 950
DO - 10.5220/0011313400003443