Determination of 4-Sulfonamidophenylhydrazine and Sulfonamide in Diarylpyrazole Derivatives by LC-MS

Meng Yu, Qing Sun, Ling Sun, Renyong Zhao, Jinhu Wang

2022

Abstract

A sensitive and selective LC-MS method was developed for the determination of 4-sulfonamidophenylhydrazine and sulfonamide in diarylpyrazole derivatives. The analysis of two impurities was done on Inertsil Ph-3 phenyl column (100 mm×4.6 mm, 3 µm). The mobile phase was gradient elution with 0.01 mol/L ammonium acetate buffer solution (pH 4.0) and methanol at a flow rate of 0.5 mL/min. Mass spectrometry adopts electrospray ion source, monitoring in positive ion mode. The limits of quantification of 4-sulfonamidophenylhydrazine and sulfonamide were 0.4915 ng/mL and 0.5079 ng/mL, respectively. They had a good linear relationship within their respective concentration ranges, and the average recoveries were 106.96% and 106.71%, respectively. The method can be used for the determination of 4-sulfonamidophenylhydrazine and sulfonamide in diarylpyrazole derivatives.

Paper Citation

in Harvard Style

Yu M., Sun Q., Sun L., Zhao R. and Wang J. (2022). Determination of 4-Sulfonamidophenylhydrazine and Sulfonamide in Diarylpyrazole Derivatives by LC-MS. In Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB, ISBN 978-989-758-595-1, pages 992-998. DOI: 10.5220/0011375300003443

in Bibtex Style

@conference{icbeb22,
author={Meng Yu and Qing Sun and Ling Sun and Renyong Zhao and Jinhu Wang},
title={Determination of 4-Sulfonamidophenylhydrazine and Sulfonamide in Diarylpyrazole Derivatives by LC-MS},
booktitle={Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,},
year={2022},
pages={992-998},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0011375300003443},
isbn={978-989-758-595-1},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biomedical Engineering and Bioinformatics - Volume 1: ICBEB,
TI - Determination of 4-Sulfonamidophenylhydrazine and Sulfonamide in Diarylpyrazole Derivatives by LC-MS
SN - 978-989-758-595-1
AU - Yu M.
AU - Sun Q.
AU - Sun L.
AU - Zhao R.
AU - Wang J.
PY - 2022
SP - 992
EP - 998
DO - 10.5220/0011375300003443