Gene Editing of Saccharomyces Cerevisiae Using CRISPR

Chenyu Yang, Yilin Li

2022

Abstract

This experiment aims to integrate the mCherry gene into saccharomyces cerevisiae (S.cerevisiae) genome and examine its expression in S.cerevisiae. This experiment is to detect whether the integration and mCherry gene expression are successful. By applying the CRISPR/Cas9 and PCR techniques, we have shown that the integration into S. cerevisiae is successful, and the mCherry gene is expressed perfectly. This experiment shows the feasibility of the CRISPR/Cas9 technique, which can be applied in clinical treatments or mass production of certain medical products.

Paper Citation

in Harvard Style

Yang C. and Li Y. (2022). Gene Editing of Saccharomyces Cerevisiae Using CRISPR. In Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB; ISBN 978-989-758-637-8, SciTePress, pages 404-409. DOI: 10.5220/0012021800003633

in Bibtex Style

@conference{icbb22,
author={Chenyu Yang and Yilin Li},
title={Gene Editing of Saccharomyces Cerevisiae Using CRISPR},
booktitle={Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB},
year={2022},
pages={404-409},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0012021800003633},
isbn={978-989-758-637-8},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB
TI - Gene Editing of Saccharomyces Cerevisiae Using CRISPR
SN - 978-989-758-637-8
AU - Yang C.
AU - Li Y.
PY - 2022
SP - 404
EP - 409
DO - 10.5220/0012021800003633
PB - SciTePress