Heterologous Expression of Fluorescent Protein Gene in E. Coli DH5α

Yilin Li, Chenyu Yang

2022

Abstract

Gene editing has opened a new field of molecular genetics research and the door for humans to understand, identify, isolate and modify genes to create new species. This project aims to produce heterologous fluorescent protein in E. coli cells by gene editing. By PCR amplification, the fluorescent protein gene has been obtained from the plasmid carrier; the target gene has been connected to the carrier, and finally transformed into the clone vector. The results of this project show that the fluorescent protein expressed by E. coli can be obtained, and the heterologous protein gene can be expressed in E. coli.

Paper Citation

in Harvard Style

Li Y. and Yang C. (2022). Heterologous Expression of Fluorescent Protein Gene in E. Coli DH5α. In Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB; ISBN 978-989-758-637-8, SciTePress, pages 410-418. DOI: 10.5220/0012025200003633

in Bibtex Style

@conference{icbb22,
author={Yilin Li and Chenyu Yang},
title={Heterologous Expression of Fluorescent Protein Gene in E. Coli DH5α},
booktitle={Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB},
year={2022},
pages={410-418},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0012025200003633},
isbn={978-989-758-637-8},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB
TI - Heterologous Expression of Fluorescent Protein Gene in E. Coli DH5α
SN - 978-989-758-637-8
AU - Li Y.
AU - Yang C.
PY - 2022
SP - 410
EP - 418
DO - 10.5220/0012025200003633
PB - SciTePress