Trypsin-Assisted Cell Depletion Method for Wound Healing Assay

Di Yin, Shihmo Yang, Hongbo Zhang, Wenjun Zhang, Wenjun Zhang, Wenjun Zhang

2022

Abstract

Wound healing assay is a commonly used method in the laboratory to study cell migration ability. Among the methods used to create cell-free zone, the widely used method, called cell depletion, will leave a certain amount of injured cells in the migration regions, which will have an impact on the subsequent healing experiments. To this end, we present a trypsin-assisted cell depletion method in wound healing assay to create cell-free zone without dead cells. This method could rinse the dead cell after applying depleting process without interfering in the attachment of the living cells. All the operation process is accomplished by commonly used equipment and drugs in biological experiments. The effect of the enzyme is controlled by the ambient temperature and processing time. The debris of dead cells are easily detached and removed to avoid the impact on wound healing assay. This method is expected to combine with other 2D and 3D cell patterning methods to form a more reliable cell processing technique.

Paper Citation

in Harvard Style

Yin D., Yang S., Zhang H. and Zhang W. (2022). Trypsin-Assisted Cell Depletion Method for Wound Healing Assay. In Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB; ISBN 978-989-758-637-8, SciTePress, pages 483-487. DOI: 10.5220/0012032800003633

in Bibtex Style

@conference{icbb22,
author={Di Yin and Shihmo Yang and Hongbo Zhang and Wenjun Zhang},
title={Trypsin-Assisted Cell Depletion Method for Wound Healing Assay},
booktitle={Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB},
year={2022},
pages={483-487},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0012032800003633},
isbn={978-989-758-637-8},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 4th International Conference on Biotechnology and Biomedicine - Volume 1: ICBB
TI - Trypsin-Assisted Cell Depletion Method for Wound Healing Assay
SN - 978-989-758-637-8
AU - Yin D.
AU - Yang S.
AU - Zhang H.
AU - Zhang W.
PY - 2022
SP - 483
EP - 487
DO - 10.5220/0012032800003633
PB - SciTePress