MILI: Biophotonics Technology for In-Situ, Fast, Accurate and Cost-Effective Milk Analysis

Panayiota Demosthenous, Maria Aspri, Maria Moyseos, Marios Sergides

2025

Abstract

Contaminated milk poses serious health risks to consumers, highlighting the need for efficient detection methods. Currently, the dairy industry relies on precise but time-consuming laboratory methods that require specialized personnel. The work presented here aims to address this by developing a fast, cost-effective and reliable system for detecting common contaminants in milk at the farm level. The MILI device is based on state-of-theart biophotonics, combining customised biosensors, optical analysis, electronics, and software modules. The detection method relies on the use of fluorometry, where the signal originating from labelled antibodies bound to specific analytes (antibiotics and toxins) is measured. Two different chromophore molecules suspended in buffer and milk solutions were used to test the detection capabilities of the device with excitation/emission wavelengths at 562 nm/584 nm and 650 nm/665 nm, respectively. We describe the different modules of the device and present a detailed experimental work performed to validate the device operation and extract performance parameters such as the limit of detection in terms of chromophore concentration, accuracy, sensitivity, and specificity. The obtained results demonstrate reliable detection of low chromophore concentrations (<100 pM), with repeatability and robustness confirmed under different conditions, making the MILI system an ideal candidate for rapid, cost-effective contamination detection device.

Paper Citation

in Harvard Style

Demosthenous P., Aspri M., Moyseos M. and Sergides M. (2025). MILI: Biophotonics Technology for In-Situ, Fast, Accurate and Cost-Effective Milk Analysis. In Proceedings of the 13th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS; ISBN 978-989-758-736-8, SciTePress, pages 64-73. DOI: 10.5220/0013130100003902

in Bibtex Style

@conference{photoptics25,
author={Panayiota Demosthenous and Maria Aspri and Maria Moyseos and Marios Sergides},
title={MILI: Biophotonics Technology for In-Situ, Fast, Accurate and Cost-Effective Milk Analysis},
booktitle={Proceedings of the 13th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS},
year={2025},
pages={64-73},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013130100003902},
isbn={978-989-758-736-8},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 13th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS
TI - MILI: Biophotonics Technology for In-Situ, Fast, Accurate and Cost-Effective Milk Analysis
SN - 978-989-758-736-8
AU - Demosthenous P.
AU - Aspri M.
AU - Moyseos M.
AU - Sergides M.
PY - 2025
SP - 64
EP - 73
DO - 10.5220/0013130100003902
PB - SciTePress