White Light Spectroscopy for Mammalian Cell Viability/Quality Assessment: Towards an Online, Label-Free and Sampling-Less System to Simplify Quality Control in CAR T-Cells Production

Bruno Wacogne, Bruno Wacogne, Céline Codjiova, Jovanne Palvair, Naïs Vaccari, Mélanie Couturier, Alain Rouleau, Annie Frelet-Barrand

2025

Abstract

CAR T-cells are highly promising medical products for personalized medicine, but their long production is complex and require extensive quality controls, which result in prohibitive costs for most patients. These controls include monitoring of cell concentration, viability, and possible contamination detection. To simplify CAR T production, these controls should ideally be conducted online in a closed system, without sampling from the bioreactor. Recently, we proposed white light spectroscopy as a method for online monitoring of cell concentration. In this paper, we demonstrate that this optical method can also assess cell viability. We define a cell suspension "quality value" which shows a linear relationship with cell viability estimated by conventional methods. This relationship varies depending on the techniques used and the dominant T-cell death process induced. We then hypothesise that the quality score could serve as a general indicator of cell suspension health, as it is not dependent on any biophysical-chemical interaction or instrument. Overall, the correlation between conventional and optical methods, together with previously published results on cell concentration monitoring, suggests that white light spectroscopy is a promising on-line and sample-free option for monitoring CAR T production.

Paper Citation

in Harvard Style

Wacogne B., Codjiova C., Palvair J., Vaccari N., Couturier M., Rouleau A. and Frelet-Barrand A. (2025). White Light Spectroscopy for Mammalian Cell Viability/Quality Assessment: Towards an Online, Label-Free and Sampling-Less System to Simplify Quality Control in CAR T-Cells Production. In Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES; ISBN 978-989-758-731-3, SciTePress, pages 53-64. DOI: 10.5220/0013149400003911

in Bibtex Style

@conference{biodevices25,
author={Bruno Wacogne and Céline Codjiova and Jovanne Palvair and Naïs Vaccari and Mélanie Couturier and Alain Rouleau and Annie Frelet-Barrand},
title={White Light Spectroscopy for Mammalian Cell Viability/Quality Assessment: Towards an Online, Label-Free and Sampling-Less System to Simplify Quality Control in CAR T-Cells Production},
booktitle={Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES},
year={2025},
pages={53-64},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013149400003911},
isbn={978-989-758-731-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES
TI - White Light Spectroscopy for Mammalian Cell Viability/Quality Assessment: Towards an Online, Label-Free and Sampling-Less System to Simplify Quality Control in CAR T-Cells Production
SN - 978-989-758-731-3
AU - Wacogne B.
AU - Codjiova C.
AU - Palvair J.
AU - Vaccari N.
AU - Couturier M.
AU - Rouleau A.
AU - Frelet-Barrand A.
PY - 2025
SP - 53
EP - 64
DO - 10.5220/0013149400003911
PB - SciTePress