Sorting Circulating Tumor Cells: A Low Flow Microfluidic Pre-Enrichment Function for Improved Separation in Serial Two-Stage Sorting Device

Emma Dupont, Emma Dupont, Emilie Laffont, Emilie Laffont, Marie Piecyk, Léa Payen, Clément Albin, Gilles Simon, Damien Le Roy, Anne-Laure Deman

2025

Abstract

The isolation of Circulating Tumor Cells (CTCs) directly from blood by liquid biopsy could lead to a paradigm shift in clinical cancer care by enabling earlier diagnosis, more accurate prognosis and personalized treatment. Nevertheless, the specific challenges of CTCs, including their rarity and heterogeneity, have so far limited the use of CTCs in clinical studies. Currently, no device fully meets the requirements of high recovery, high purity, short processing time and ease of use for end-users. A promising new strategy involves combining a higher throughput but less specific pre-enrichment step based on size sorting together with a highly specific but slower immunomagnetic sorting. This approach requires the initial function to operate at lower flow rates than commonly used to connect the two functions in series. In this context, we developed a Dean spiral microfluidic device, optimized for sorting 10µm and 15µm beads by size. We showed that it successfully separates mimicking CTCs from white blood cells at low flow rates (<100 mL/h).

Paper Citation

in Harvard Style

Dupont E., Laffont E., Piecyk M., Payen L., Albin C., Simon G., Roy D. and Deman A. (2025). Sorting Circulating Tumor Cells: A Low Flow Microfluidic Pre-Enrichment Function for Improved Separation in Serial Two-Stage Sorting Device. In Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES; ISBN 978-989-758-731-3, SciTePress, pages 163-170. DOI: 10.5220/0013162200003911

in Bibtex Style

@conference{biodevices25,
author={Emma Dupont and Emilie Laffont and Marie Piecyk and Léa Payen and Clément Albin and Gilles Simon and Damien Roy and Anne-Laure Deman},
title={Sorting Circulating Tumor Cells: A Low Flow Microfluidic Pre-Enrichment Function for Improved Separation in Serial Two-Stage Sorting Device},
booktitle={Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES},
year={2025},
pages={163-170},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013162200003911},
isbn={978-989-758-731-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES
TI - Sorting Circulating Tumor Cells: A Low Flow Microfluidic Pre-Enrichment Function for Improved Separation in Serial Two-Stage Sorting Device
SN - 978-989-758-731-3
AU - Dupont E.
AU - Laffont E.
AU - Piecyk M.
AU - Payen L.
AU - Albin C.
AU - Simon G.
AU - Roy D.
AU - Deman A.
PY - 2025
SP - 163
EP - 170
DO - 10.5220/0013162200003911
PB - SciTePress