Development of WHO Guideline-Complying CD4 Diagnostic Chip

Hee Sik Shin, Sungyoung Choi

2025

Abstract

Accurate HIV diagnosis using current WHO complying diagnostic chips that measure CD4 protein expression faces challenges due to donor variability in expression levels and difficulties in isolating target cells with high purity. To overcome these limitations, quantitative measurement of CD4+ and CD8+ T cells, along with the effective removal of red blood cells (RBCs) and granulocytes, is essential. We present a self-powered CD4 diagnostic chip platform designed for high0purity target cell separation using a small volume of whole blood. The CD4 diagnostic chip employs magnetic separation to remove non-target cells through negative selection, utilizing ferromagnetic particle-filled inlets and a magnet positioned beneath the inlet. Excessive RBCs and target cells are further separated by size using a microfluidic lattice. This rapid separation process, facilitated by a degassed polydimethylsiloxane (PDMS) chip, achieves efficient target cell isolation within 20 minutes. During separation, the LEGO-integrated smartphone microscope records a real-time video, which is analyzed using Python-based code. The code distinguishes and removes excess granulocytes based on pixel intensity and precisely counts pure target cells, enabling analysis and potential diagnosis within 1 minute post-separation. The cD4 diagnostic chip is a simple, precise, and rapid platform requiring minimal blood volume, compliant with WHO guidelines for HIV diagnosis.

Paper Citation

in Harvard Style

Shin H. and Choi S. (2025). Development of WHO Guideline-Complying CD4 Diagnostic Chip. In Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES; ISBN 978-989-758-731-3, SciTePress, pages 175-179. DOI: 10.5220/0013254700003911

in Bibtex Style

@conference{biodevices25,
author={Hee Shin and Sungyoung Choi},
title={Development of WHO Guideline-Complying CD4 Diagnostic Chip},
booktitle={Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES},
year={2025},
pages={175-179},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013254700003911},
isbn={978-989-758-731-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES
TI - Development of WHO Guideline-Complying CD4 Diagnostic Chip
SN - 978-989-758-731-3
AU - Shin H.
AU - Choi S.
PY - 2025
SP - 175
EP - 179
DO - 10.5220/0013254700003911
PB - SciTePress