Exploring Endothelial Cell Adhesion to High-Resolution 3D Printing Materials for Advanced Organ-on-Chip Fabrication

Steffen Winkler, Xenia Kraus, Jasmin Huber, Janina Bahnemann, Janina Bahnemann

2025

Abstract

With advancements in resolution, 3D printing is emerging as a transformative technology for the rapid fabrica-tion of cell culture systems, including organ-on-chip platforms. For successful integration into cell culture en-vironments, 3D printing materials must not only exhibit general biocompatibility but also support direct cell adhesion for on-chip cultivation. In this study, we investigated the adhesion of human umbilical vein endothe-lial cells (HUVECs) to two 3D printing materials, AR-M2 and M2S-HT90, under varying sterilization condi-tions involving heat steam sterilization and ethanol disinfection. Our findings reveal that specific combinations of these sterilization techniques significantly enhance cell adhesion, achieving levels comparable to standard cell culture plates. However, alterations in the 3D printing mode resulted in a complete loss of cell adhesion, underscoring the critical impact of printing parameters on the material surface properties.

Paper Citation

in Harvard Style

Winkler S., Kraus X., Huber J. and Bahnemann J. (2025). Exploring Endothelial Cell Adhesion to High-Resolution 3D Printing Materials for Advanced Organ-on-Chip Fabrication. In Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: microOrganChip; ISBN 978-989-758-731-3, SciTePress, pages 1063-1067. DOI: 10.5220/0013418600003911

in Bibtex Style

@conference{microorganchip25,
author={Steffen Winkler and Xenia Kraus and Jasmin Huber and Janina Bahnemann},
title={Exploring Endothelial Cell Adhesion to High-Resolution 3D Printing Materials for Advanced Organ-on-Chip Fabrication},
booktitle={Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: microOrganChip},
year={2025},
pages={1063-1067},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0013418600003911},
isbn={978-989-758-731-3},
}

in EndNote Style

TY - CONF

JO - Proceedings of the 18th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: microOrganChip
TI - Exploring Endothelial Cell Adhesion to High-Resolution 3D Printing Materials for Advanced Organ-on-Chip Fabrication
SN - 978-989-758-731-3
AU - Winkler S.
AU - Kraus X.
AU - Huber J.
AU - Bahnemann J.
PY - 2025
SP - 1063
EP - 1067
DO - 10.5220/0013418600003911
PB - SciTePress