Ex-vivo Platelet Activation using Electric Pulse Stimulation

Nicole LaPlante, V. Bogdan Neculaes, Brian D. Lee, Andrew S. Torres, Kenneth Conway, Steve Klopman, Antonio Caiafa

2013

Abstract

Activated platelet rich plasma (PRP), also known as platelet gel, is an encouraging autologous cell therapy with numerous applications in areas including: wound healing, haemostasis and wound infection control. Activation of PRP using electric pulse stimulation is a promising alternative to activation via biologics such as bovine thrombin. By removing the need for biologics, it is possible to deliver a cost-effective, fast, truly autologous platelet gel option. In this position paper, we describe parameters for effective ex-vivo release of several growth factors from human platelets in PRP using electric field pulses with the duration of hundreds of nanoseconds. Growth factor release levels with nanosecond pulse electric fields seem at the same level or higher compared to bovine thrombin, the standard platelet activator used in clinical practice. These findings suggest that electric pulse stimulation has the potential to become not only a viable alternative to biochemical platelet activators, but to actually enhance the desired in vivo biological effects, such as wound healing.

References

  1. Tate, K. S. and Crane, D. M., 2010. Platelet rich plasma grafts in musculoskeletal medicine. In Journal of Prolotherapy, Volume 2, Issue 2.
  2. Driver, V. R., Hanft, J., Fylling, C. P., Beriou, J. M., 2006. A prospective, randomized, controlled trial of autologous platelet-rich plasma gel for the treatment of diabetic foot ulcers. In Ostomy/Wound Management, 52(6).
  3. Lacci, K. M. and Dardik, A., 2010. Platelet-rich plasma : support for its use in wound healing. In Yale Journal of biology and medicine, 83.
  4. Gunaydin, S., McCusker, K., Sari, T., Onur, M., Gurpinar, A., Sevim, H., Atasoy, P., Yorgancioglu, C., Zorlutuna Y., 2008. Clinical impact and biomaterial evaluation of autologous platelet gel in cardiac surgery. In Perfusion, 23, 179-186.
  5. Alexander, W., 2009. Meeting highlights of the 30th American College of Clinical Pharmacy annual meeting. In Pharmacy & Therapeutics, vol. 34, no. 12.
  6. Cada, D. J., Levien, T. Baker, D. E., 2008. Thrombin, Topical (Recombinant). In Hospital Pharmacy Volume 43, Number 7, Wolters Kluwer Health, Inc.
  7. Beebe, S. J. and Schoenbach, K. H., 2005. Nanosecondpulsed electric fields: a new stimulus to activate intracellular signalling. In Journal of Biomedicine and Biotechnology. 4:297-300.
  8. Zhang, J., Blackmore, P. F., Hargrave, B. Y., Xiao, S., Beebe, S.J, Schoenbach, K.H., 2008. Nanosecond electric field pulse (nanopulse): a novel non-ligand agonist for platelet activation. In Archives of biochemistry and biophysics, 417.
  9. Whitlow, J., Shackelford, A. G., Sievert, A. N., Sistino, J. J., 2008. Barriers to the acceptance and use of autologous platelet gel. In Perfusion, 23: 283-289
  10. Everts, P. A. M., Mahoney, C.B., Hoffmann, J.J.M.L., Schonberger, J. P. A. M., Box, H. A. M., van Zundert, A., Knape, J. T. A., 2006. Platelet rich plasma preparation using three devices: Implications on platelet activation and platelet growth factor release. In Growth Factors. 24:165-171.
  11. Maloney, J. P., Silliman, C.C., Ambruso, D. R., Wang, J., Tuder, R. M., Voelkel, N.F., 1998. In vitro release of vascular endothelial growth factor during platelet aggregation. In American Journal of PhysiologyHeart and Circulatory Physiology 275:H1054-H1061.
  12. Poliachik, S. L., Chandler, W. L., Mourad, P. D., Ollos, R. J., and Crum, L. A., 2001. Activation, aggregation and adhesion of platelets exposed to high-intensity focused ultrasound. In Ultrasound in Medicine and Biology, Vol. 27, No. 11, pp. 1567-1576.
  13. Verhaar, R., Dekkers, D. W. C., De Cuyper, I. M., Ginsberg, M. H., Korte, D. Verhoeven, A. J., 2008. UV-C irradiation disrupts platelet surface disulfide bonds and activates the platelet integrin _IIb_3. In Blood, Volume 112, Number 13
  14. Mazzucco, L., Balbo, V., Cattana, E., Guaschino, R., and Borzini, P., 2009. Not every PRP-gel is born equal - Evaluation of growth factor availability for tissues through four PRP-gel preparations: Fibrinet®, RegenPRP-Kit®, Plateltex® and one manual procedure. In Vox Sanguinis 97, 110-118
Download


Paper Citation


in Harvard Style

LaPlante N., Bogdan Neculaes V., D. Lee B., S. Torres A., Conway K., Klopman S. and Caiafa A. (2013). Ex-vivo Platelet Activation using Electric Pulse Stimulation . In Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013) ISBN 978-989-8565-34-1, pages 202-208. DOI: 10.5220/0004328602020208


in Bibtex Style

@conference{biodevices13,
author={Nicole LaPlante and V. Bogdan Neculaes and Brian D. Lee and Andrew S. Torres and Kenneth Conway and Steve Klopman and Antonio Caiafa},
title={Ex-vivo Platelet Activation using Electric Pulse Stimulation},
booktitle={Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013)},
year={2013},
pages={202-208},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0004328602020208},
isbn={978-989-8565-34-1},
}


in EndNote Style

TY - CONF
JO - Proceedings of the International Conference on Biomedical Electronics and Devices - Volume 1: BIODEVICES, (BIOSTEC 2013)
TI - Ex-vivo Platelet Activation using Electric Pulse Stimulation
SN - 978-989-8565-34-1
AU - LaPlante N.
AU - Bogdan Neculaes V.
AU - D. Lee B.
AU - S. Torres A.
AU - Conway K.
AU - Klopman S.
AU - Caiafa A.
PY - 2013
SP - 202
EP - 208
DO - 10.5220/0004328602020208